Figure 5.
Figure 5 Structure of the E188A Bh-RNase HC product complex. (A)
Stereo view of the active site superimposed with the 2F[o]-F[c]
map contoured at 0.9 .
The 5'-phosphate is shown as red and yellow ball-and-sticks,
Mg^2+ ions as purple spheres labeled with 'A' and 'B', and water
molecules as small red spheres. (B, C) Side-by-side comparison
of metal ion coordination in the substrate and product
complexes. The substrate complex (D192N, PDB: 1ZBL) is shown in
green with oxygen atoms in red and nitrogen atoms in blue, and
the product complex (E188A) is shown in orange. (D) Stereo view
of the superposition of substrate and product complexes.
The above figure is reprinted
by permission from Macmillan Publishers Ltd:
EMBO J
(2006,
25,
1924-1933)
copyright 2006.
.
The 5'-phosphate is shown as red and yellow ball-and-sticks,
Mg^2+ ions as purple spheres labeled with 'A' and 'B', and water
molecules as small red spheres. (B, C) Side-by-side comparison
of metal ion coordination in the substrate and product
complexes. The substrate complex (D192N, PDB: 1ZBL) is shown in
green with oxygen atoms in red and nitrogen atoms in blue, and
the product complex (E188A) is shown in orange. (D) Stereo view
of the superposition of substrate and product complexes.