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Figure 5.
Figure 5. The MIU:Ub Complex Resembles the UIM:Ub Complex
(A) Structures of the Rabex-5-MIU:Ub (yellow and green) and
the Vps27-UIM:Ub (red and green) complexes.
(B) 2F[obs] −
1F[calc] difference electron density contoured at the 1σ level
around residue Ala58[Rbx] (yellow) and residues Ile44[Ub] and
Val70[Ub] (green).
(C) Interactions between Ub (green) and
MIU (yellow). Hydrogen bonds and salt bridges are indicated by
dashed black lines.
(D) Ub binding residues of the MIU of
Rabex-5 (yellow) and of the UIM of Vps27 (red). Models of the
RZF-MIU:Ub complex and the Vps27-UIM:Ub complex (Swanson et al.,
2003) were superimposed based on residues 5–70 of the Ub
molecules. Side chains involved in interaction between MIU
(yellow)/UIM (red) and Ub are shown in stick representation.
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