Figure 5.
Figure 5 Lipid-binding specificity of the PH[N]-PDZ-PH[C]
supramodule using phospholipid strip overlay assay. (A) Binding
of the PH[N]-PDZ-PH[C] tandem to the phospholipids spotted on a
cellulose membrane. The amount of lipid per spot was 100 pmol.
Abbreviations for the lipids: S1P: sphingosine-1-phosphate; LPA:
lysophosphatidic acid; LPC: lysophosphocholine; PE:
phosphatidylethanolamine; PS: phosphatidylserine; PA:
phosphatidic acid; PC: phosphatidylcholine; PtdIns:
phosphatidylinositol. The amount of protein used in the assay is
0.5 g/ml.
(B) Binding isotherms of PH[N]-PDZ-PH[C] with PC/PS liposomes
containing either 10 or 20% PI(3,5)P[2]. (C) Identification of
the residues in the split PH domain of the PH[N]-PDZ-PH[C]
tandem that are involved in the lipid binding.
The above figure is reprinted
by permission from Macmillan Publishers Ltd:
Embo J
(2005,
24,
3985-3995)
copyright 2005.
g/ml.
(B) Binding isotherms of PH[N]-PDZ-PH[C] with PC/PS liposomes
containing either 10 or 20% PI(3,5)P[2]. (C) Identification of
the residues in the split PH domain of the PH[N]-PDZ-PH[C]
tandem that are involved in the lipid binding.