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Figure 5.
Fig. 5. Calcium dependence of other Q425-like Abs binding
to CD4. CD4 was immobilized on a CM5 chip, and the binding of
Q425, Q428, and Q4116 IgG was tested. Sensorgrams are shown for
Q425, Q428, and Q4116 in the presence of 2.5 mM Ca^2+ and also
in the presence of 1 mM EDTA. IgG (144 nM) was passed over the
chip starting at 80 s and allowed to associate for 170 s, with
the dissociation followed for 5 min.
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