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Figure 5.
Figure 5 Chromatogram of the gel filtration step for the RecOR
complex using a flow rate of 0.5 ml/min. The green and violet
lines show the absorbance at 280 and 260 nm, respectively. The
inset is the SDS -PAGE denaturing gel of the fractions as shown
above the chromatogram. Molecular weight markers (M) are shown
in kDa. drRecR (23.7 kDa) migrates as being slightly larger than
drRecO (26.3 kDa) and the proteins are at an apparent 2:1 ratio
in the RecOR complex.
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