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Figure 5.
Figure 5 The inhibited phosphorylation of MBP, the docking
site-independent substrate, due to the reduced ATP binding
affinity to JNK1 by pepJIP1 binding. (A, B) The binding
affinities of ATP to JNK1 were measured by ITC when pepJIP1 was
unbound (A) and bound (B) to JNK1. (C) Dose-dependent inhibition
of the kinase activity of JNK1 by pepJIP1 using MBP as
substrates. The mutated pepJIP1 used for control experiment has
the sequence of RPKAATTANAF.
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