Figure 6.
Figure 6. C Lobe and N Lobe Interactions in WWP1/AIP5(A)
Residue contacts between the N and C lobes within WWP1/AIP5 in
the shape
conformation. Asp793 on the N lobe interacts with Arg845 on the
C lobe. Gln848 and Arg855 on the C lobe form hydrogen bonds with
Glu798 and the carbonyl oxygen of Thr676. Asp793 and Arg845 are
highly conserved, and Glu798, Gln848, and Arg855 are absolutely
conserved among 22 HECT domain sequences analyzed (data not
shown).(B) Ubiquitin transfer assays.
The above figure is reprinted
by permission from Cell Press:
Mol Cell
(2003,
11,
249-259)
copyright 2003.
shape
conformation. Asp793 on the N lobe interacts with Arg845 on the
C lobe. Gln848 and Arg855 on the C lobe form hydrogen bonds with
Glu798 and the carbonyl oxygen of Thr676. Asp793 and Arg845 are
highly conserved, and Glu798, Gln848, and Arg855 are absolutely
conserved among 22 HECT domain sequences analyzed (data not
shown).(B) Ubiquitin transfer assays.