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Figure 5.
Figure 5. Superposition of representative regions of the
1H, 15N correlation spectra for the interaction of the Prp40
tandem WW domains and the second Rsp5 WW domain with different
proline-rich peptides. The free WW domains are shown in black
(reference spectra without ligand). In (a)-(c), G* corresponds
to a glycine residue resulting from the TEV protease cleavage
site (see Materials and Methods). (a) Addition of PPxY/F motif
containing peptides from BBP Image in green) and Prp8 Image in
blue and Image in red) to the Prp40 tandem WW domains. (b)
Addition of PPQQP motif containing peptides from mouse formin
Image in blue) and the Abl-SH3 3BP-10 peptide Image in red) to
the Prp40 tandem WW domains. (c) Addition of Prp8 peptide
(PPPPSNFE in green), the unphosphorylated tandem CTD repeat
(YSPTSPSYSPTSPS in blue) and the doubly phosphorylated CTD
repeat (SYpSPTpSPS in red) to the Prp40 tandem WW domains. (d)
Addition of the unphosphorylated tandem CTD repeat
(YSPTSPSYSPTSPS in red) and the doubly phosphorylated CTD repeat
(SYpSPTpSPS in cyan) to the second WW domain of Rsp5. All
peptide/protein ratios refer to the WW domain pair for Prp40 and
to the single domain for Rsp5.
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