Figure 5.
Figure 5 Hin−DNA interactions in models of T11A and A10T. (A)
Energy-minimized model of T11A. The T21 and T22 methyl groups
are rendered as van der Waals spheres. The T21-methyl occludes
W2 but may stabilize W1. (B) Substitution of a T:A base pair at
position 10 in mutant A10T results in a clash with the Ser174
side chain. The van der Waals surfaces of T10−C5A and Ser174-O
are
rendered. Substitution of a C:G base pair in mutant A10C also
results in a clash with the Ser174 side chain (not shown). Note
that saturable binding was never achieved with A10T or A10C,
even at native Hin or Hin-DBD concentrations exceeding 1 M.
Models were generated by visually fitting the substituted base
pair into the Br18 structure, and for T11A, energy minimized
using CNS (Brünger et al., 1998).
The above figure is reprinted
from an Open Access publication published by Macmillan Publishers Ltd:
EMBO J
(2002,
21,
801-814)
copyright 2002.
are
rendered. Substitution of a C:G base pair in mutant A10C also
results in a clash with the Ser174 side chain (not shown). Note
that saturable binding was never achieved with A10T or A10C,
even at native Hin or Hin-DBD concentrations exceeding 1 
M.
Models were generated by visually fitting the substituted base
pair into the Br18 structure, and for T11A, energy minimized
using CNS (Brünger et al., 1998).