Figure 5.
Figure 5: The active site of EF and its comparison with mAC.
a, Ball-and-stick representation of the EF active site. b,
Proposed mechanism of catalysis of EF. For clarity, only
residues that are directly involved in 3' O- to P nucleophilic
attack are shown. c, Ball-and-stick representation of the mAC
active site. d, Secondary structures of the EF catalytic core
and the mAC catalytic core with the same view as in a and c. The
nucleotide (3'dATP in EF -CaM and ATP- S
in mAC) and the metal ion are in black and grey, respectively.
The O4', C4' and P of
ATP- S
are shown in a similar position to those of 3'dATP in a for
comparison. C1a and C2a of mAC are in green and yellow,
respectively.
The above figure is reprinted
by permission from Macmillan Publishers Ltd:
Nature
(2002,
415,
396-402)
copyright 2002.
nucleophilic
attack are shown. c, Ball-and-stick representation of the mAC
active site. d, Secondary structures of the EF catalytic core
and the mAC catalytic core with the same view as in a and c. The
nucleotide (3'dATP in EF -CaM and ATP-