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Figure 5.
Figure 5. A Schematic of the Proposed Reaction Mechanism of
MALThe 3-proton of (2S,3S)-3-methyl aspartic acid is abstracted
by Lys-331 acting as a base to give the enolic intermediate
shown in the middle panel. The negative charge on the
aci-carboxylate is stabilized by the metal ion and possibly by
His-194 acting as an electrophile. The enolic intermediate
collapses with the elimination of ammonia to yield mesaconic
acid (right hand panel).
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