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Figure 5.
Figure 5. A ribbon diagram showing the secondary structure of EGF-AB. The b-strands are shown with yellow
arrows. Two calcium ion sites, located near the N terminus of EGF-A and in the intermodule interface of EGF-AB,
are shown with white spheres. The N-terminal EGF-A binding site consists of the carboxyl side-chains of E4 and
D18. The aromatic ring of Y23 is involved in calcium binding indirectly by shielding the site from the solvent. The
intermodule EGF-AB binding site consists of the backbone carbonyl groups of I42 and L58, the carboxyl side-chains
of D41 and E44, and the carbonyl side-chain of N57. The aromatic ring of F31 forms an intermodule interaction with
residues E59, G60 and G61 (not labeled for clarity). Y62, which is sequentially equivalent to Y23, is also in close
proximity to the calcium ion site but appears not to be involved in the binding. This diagram was produced using
Insight98 (Molecular Simulations Inc.).
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