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Figure 5.
Figure 5. Model of the Interactions of the PapGII Adhesin
at the Membrane(A) A P pilus. The PapG adhesin is located at the
thin tip of the pilus. Note the bend at the tip of the pilus,
which places the PapG adhesin in a proper orientation for
side-on binding to the membrane.(B) Model of PapGII receptor
binding domain interacting with GbO4-ceramide. This model was
generated by attaching a ceramide group to the Galβ1-4Glc
moiety of GbO4 such that the resulting Galβ1-4Glcβ-ceramide
would adopt a configuration similar to that crystallographically
observed for a digalactoside-ceramide (Pascher et al., 1992).
The molecular surface is that of the protein, color-coded
according to charge, blue for positive (Arg and Lys) and red for
negative (Glu and Asp). Charged residues which could potentially
interact with the head groups of the eukaryotic membrane are
labeled. The GbO4-ceramide is in CPK representation with carbon
atoms in green, oxygen atoms in red, and nitrogen atoms in
blue.(C) Model of uroepithelium. The GbO4-ceramide is
represented as in (B) and is labeled “GbO4”
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