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Figure 6.
Figure 6. Structure of the P-LE/SGP-3 Complex and Binding
Interactions Involving Tys Residues(A) Ribbon/stick
representation of the P-LE/SGP-3 structure superimposed on the
unliganded structure of P-LE. The unliganded structure of P-LE
is shown in blue, complexed P-LE in purple, and SGP-3 in orange.
The bound strontium ion in the P-LE/SGP-3 complex is directly
superimposed over the calcium ion in unliganded P-LE and is
shown as a green sphere. The MPD molecule that is found at the
lectin-EGF domain interface in the P-LE/SGP-3 complex is shown
in dark blue.(B) Stereo view of a close-up of P-LE/SGP-3
interactions in the region of Tys7 (in orange) illustrating the
hydrogen bonding network with P-LE (purple). The sulfur atom in
Tys7 is shown in yellow.(C) Stereo view of a close-up of the
P-LE/SGP-3 interaction in the region of Tys10 and the Fuc
binding site illustrating the change in conformation and binding
contacts for the Asn83 to Asp89 loop within P-LE. Uncomplexed
P-LE is shown in blue and P-LE complexed with SGP-3 is shown in
purple. SGP-3 residues are shown in orange (the sulfur atom of
Tys10 is in yellow) and the bound strontium ion is shown as a
green sphere. The portion of SGP-3 omitted for clarity is shown
as an orange ellipse.
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