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Figure 5.
Figure 5. The Putative Substrate Binding Pocket of MobA(a)
Ribbon diagram showing the locations of strictly conserved
surface residues. The diagram was produced with MOLSCRIPT [53]
and Raster3D [54] (blue = nitrogen, RED = oxygen, YELLOW =
carbon).(b) Molecular surface displaying degrees of sequence
identity for residues in and around the pocket according to the
alignment shown in Figure 4. Warm colors indicate a high level
of sequence identity, while cold colors indicate little or no
conservation. Strictly conserved residues are shown in red.(b)
Electrostatic surface potentials of MobA. Potentials of less
than -10 kT, neutral, and greater than 10 kT are displayed in
red, white, and blue, respectively.Surfaces for (a) and (b) were
calculated with SwissPDBviewer [55] (http://www.expasy.ch/spdbv)
and rendered using POV-Ray (http://www.povray.org). In order to
get a more accurate impression of the surface conservations and
charge, those side chains that could not be confidently placed
in electron density were inserted and modeled into energetically
favorable conformations. This was necessary for only one
strictly conserved residue, namely Arg-19. All parts of this
figure display the same view of the molecule. The white line
delineates the proposed substrate binding pocket, and the labels
A-D are for use in the discussion
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