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Figure 5.
Figure 5. Active site of mODC′ and comparison with ALR. (a)
Schematic drawing of the mODC′ active site illustrating the
hydrogen-bond interactions. Residues shown in bold face are
nearer the viewer. (b) Stereo figure of the active site of
mODC′ with electron density superimposed with its model. K69
of mODC′ is in Schiff-base linkage to the cofactor, E274 pairs
with the pyridine ring nitrogen N1, and H197 stacks on the si
face of the cofactor ring. Note the angle between K69 and the
pyridine ring of the cofactor exposing the si face. The map is a
2F[o]–F[c] map at 1.6 Å resolution contoured at 1.2σ.
(c) A view of the ALR and mODC′ active sites resulting from
the superposition of their cofactor rings. The mODC′ active
site is depicted in light gray. The figures were generated using
BOBSCRIPT [83], MOLSCRIPT [80] and Raster3D [81].
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