|
Figure 4.
Figure 4. (a) Stereoview of a difference Fourier m(F[obs] -
F[calc]) map showing the electron density of HBY 097 in the
wild-type HIV-1 RT/HBY 097 complex. The map is calculated at 3.1
Å resolution with 2s contours (in magenta). The phases
were computed from the protein model prior to inclusion of the
inhibitor. The green density corresponds to the difference
Fourier map (3.7 Å resolution) between HBY 097 and S-0483
complexed with wild-type HIV-1 RT (bromine in S-0483 replaces
the methoxy group of HBY 097), contoured at the 5s level,
showing the position of the bromine atom and confirming the
orientation and placement of the inhibitor. Difference Fourier
2mF[obs] - F[calc] map at 3.3 Å resolution, contoured at
1.2s, (b) of the Tyr188Leu mutant HIV-1 RT/HBY 097 complex at
the NNIBP region in p66 showing the absence of any density for
the side-chain of Leu188; clear density for HBY 097 is seen in
the binding pocket; and of a (c) similar region in the p51
subunit, showing clear electron density for the side-chain at
Leu188.
|
