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Figure 4.
Fig. 4. Active site of subtilisin Carlsberg in anhydrous
dioxane compared with that in water (A) and acetonitrile (B).
The catalytic^ triad, Asn-155 of the oxyanion hole (OH), and
solvent molecules in the dioxane structure are shown as
balls-and-sticks with carbon, oxygen, and nitrogen shown in
white, light-gray, and black, respectively. The water molecules
in the aqueous and acetonitrile structures are depicted as
dark-gray balls in A and B. The acetonitrile molecules in that
structure (B) are also shown as linear balls-and-sticks. The
surface of the protein [Connolly algorithm (20)] in the^
active-site region in the dioxane structure is portrayed by
black dots.
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