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Figure 4.
Fig. 4. Van der Waals surface diagram ofthe residues aroundthe
structural water in the WT (A) an the D99N mutant (B). Notice
that the new position and conformation of la-1 in B fiis the void
created by the missing structural water. C: Overlay fthe skele-
tal drawings ofthe WT (red) and hemtant D99N (green) show-
ing the hydrogen bonding scheme. Residues around Ala-I that are
hydrogen bonded to theammoniumgroup are also Notice
the large change in theorientationofthe NH3+and p
groups of Ala-1. Despite this change, theammonium group in the
mutant still forms 3 hydrogen bonds, 2 f which areto thesame
acceptorsas in he WT.
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