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Figure 4.
KIAA1718 selectively demethylates H3K27me2 in the presence of
H3K4me3 in cis.
(a) A model of KIAA1718 PHD on methylated
H3K4 and its linked jumonji active site on a target lysine
(left). Surface representation displayed as blue for positive,
red for negative and white for neutral (right). The dashed line
connects H3K4me3 bound in the aromatic cage and the target
lysine in the jumonji domain. (b) The presence of H3K4
methylation in cis enhances KIAA1718 demethylase activities on
H3K27me2. (c) When two peptide substrates were mixed in
equimolar ratio, H3[1–35]K27me2 (left) and
H3[1–35]K4me3-K27me2 (right), KIAA1718 selectively
demethylated H3[1–35] peptides containing both H3K4me3 and
H3K27me2 (right).
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