|
Figure 4.
Effect of blocking Ca^2+ binding to a single lobe on the
K[D][,app] of CaM for Ca^2+ when bound to peptide. All data were
collected in triplicate as the mean ± S.D. A, 1 μm
F19W/E34Q alone (closed circles) or with 5 μm Ca[V]1.1 IQ (open
circles) or with 5 μm Ca[V]1.2 IQ (closed diamonds). All data
are fit with standard sigmoid curve. Long dashes, fit for
F19W/Ca[V]1.2 IQ shown previously (1); dash-dot-dot, fit for
F19W/Ca[V]1.1 IQ from Fig. 3. B, 1 μm F92W/E12Q alone (closed
circles) or with 5 μm Ca[V]1.1 IQ (open circles) are plotted.
Long dashes, fit for F92W/Ca[V]1.2 IQ shown previously (1);
dash-dot-dot, fit for F92W/Ca[V]1.1 IQ from Fig. 3; dotted line,
fit for F92WE12Q/Ca[V]1.2 IQ shown previously (1).
|
