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Figure 4.
Protein-ligand interaction. The NADP^+ molecule is shown as
yellow stick, and the substrate is displayed as green stick.
Water molecules are shown as red spheres and metal ions as
magenta spheres. Hydrogen bonds are shown as dotted black lines.
(A) The interaction between NADP^+ and nucleotide-binding motif
GXXXGXG. Of note is that Tyr24, not a basic residue, forms
hydrogen bond contact with 2[prime prime or minute]-phosphate
group of the adenine ribose. (B) Stereo view of a detailed
network of hydrogen bonds formed by Ga5DH and its ligands. The
substrate and cofactor are fixed in a plausible position
favorable for the subsequent dehydrogenation reaction. The
catalytically important residues Tyr163 and Lys167 are shown as
cyan stick.
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