Figure 4.
Comparison of the CCCH-type zinc finger motif structures of
TIS11d and MBNL2. (A) Superposition of the ribbon diagrams of
the TZF12 and TZF34 domains of MBNL2 with the ligand residue
side-chains and zinc ions. (B) Superposition of the ribbon
diagrams of ZF1 and ZF2 of TIS11d and ZF1-4 of MBNL2 with the
ligand residue side-chains and zinc ions. ZF1 and ZF2 of TIS11d
are shown in green and blue, respectively. The colors of ZF1-ZF4
of MBNL2 are the same as in Figures 2 Figure 2-and
and3.3 Figure 3-.
(C) Expanded view of the pocket region with the side chains of
the conserved residues. The colors of the side chains are the
same as in D --F. (D --F) Ribbon presentations with the side
chains of the conserved positively charged and aromatic residues
for ZF1 of TIS11d (D), ZF1 of MBNL2 (E), and ZF2 of MBNL2 (F).
(G --L) Surface representations showing hydrophobic and
positively charged residues of the ZF1-RNA complex of TIS11d
(G), ZF1 of MBNL2 (H), ZF2 of MBNL2 (I), the ZF2-RNA complex of
TIS11d (J), ZF3 of MBNL2 (K), and ZF4 of MBNL2 (L). The RNA is
shown in yellow in (G) and (J). Positively charged residues are
colored blue, and aliphatic and aromatic hydrophobic residues
are in green and magenta, respectively. The cyan and red
ellipses mark pockets 1-2 and pocket 3, respectively. (M, N)
Surface superposition of the ZF1 complexed with RNA in TIS11d
with ZF1 (M) and ZF2 (N) of the TZF12 in MBNL2. The surfaces of
the ZF1 of TIS11d, and of ZF1 and ZF2 of TZF12 in MBNL2 are
colored green, light blue, and orange, respectively. The
conserved residues of the ZF1 in TIS11d and the TZF12 in MBNL2
with the red annotations are colored blue and red, respectively.
The RNA is depicted by yellow sticks. Figures M and N were
generated by PyMOL program (DeLano Scientific LLC).
The above figure is reprinted
from an Open Access publication published by the Protein Society:
Protein Sci
(2009,
18,
80-91)
copyright 2009.
and
and3.3 Figure 3-
.
(C) Expanded view of the pocket region with the side chains of
the conserved residues. The colors of the side chains are the
same as in D --F. (D --F) Ribbon presentations with the side
chains of the conserved positively charged and aromatic residues
for ZF1 of TIS11d (D), ZF1 of MBNL2 (E), and ZF2 of MBNL2 (F).
(G --L) Surface representations showing hydrophobic and
positively charged residues of the ZF1-RNA complex of TIS11d
(G), ZF1 of MBNL2 (H), ZF2 of MBNL2 (I), the ZF2-RNA complex of
TIS11d (J), ZF3 of MBNL2 (K), and ZF4 of MBNL2 (L). The RNA is
shown in yellow in (G) and (J). Positively charged residues are
colored blue, and aliphatic and aromatic hydrophobic residues
are in green and magenta, respectively. The cyan and red
ellipses mark pockets 1-2 and pocket 3, respectively. (M, N)
Surface superposition of the ZF1 complexed with RNA in TIS11d
with ZF1 (M) and ZF2 (N) of the TZF12 in MBNL2. The surfaces of
the ZF1 of TIS11d, and of ZF1 and ZF2 of TZF12 in MBNL2 are
colored green, light blue, and orange, respectively. The
conserved residues of the ZF1 in TIS11d and the TZF12 in MBNL2
with the red annotations are colored blue and red, respectively.
The RNA is depicted by yellow sticks. Figures M and N were
generated by PyMOL program (DeLano Scientific LLC).