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Figure 4.
Figure 4. Protein DNA Contacts in Wild-Type and N141A
HincII/DNA Structures
(A) Stereo view of the contacts to
the DNA by residues 138–141 of N141A HincII. Colored by atom
type: N-blue, O-red, P-orange, C-green in protein, pink in DNA
of the recognition site and yellow in flanking base pairs. A
water molecule is shown as a red sphere. Dashes indicate
hydrogen bonds.
(B) Stereo view of the contacts to the DNA
by residues 138–141 of wild-type HincII. Colored as in (A).
Dashes indicate hydrogen bonds.
(C) Stereo view of the
contacts to the DNA at Thy 2′ and Gua 1′ of N141A HincII.
Colored as in (A). Methyl groups are shown as green or pink
spheres; water molecule is shown as a red sphere. Dashes
indicate hydrogen bonds or van der Waals interactions.
(D)
Stereo view of the contacts to the DNA at Thy 2′ and Gua 1′
of wild-type HincII. Colored as in (A). Methyl groups are shown
as green or pink spheres. Dashes indicate hydrogen bonds or van
der Waals interactions.
(E) Cartoon of protein-DNA contacts
to bases in the major groove in the N141A HincII/DNA structure.
Water molecule is shown as a red circle.
(F) Cartoon of
protein-DNA contacts to bases in the major groove in the
wild-type HincII/DNA structure. Dashes indicate hydrogen bonds
or van der Waals interactions.
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