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Figure 4.
Overlay of the combining sites of 1E9, 1E9dm, and DB3 with
bound ligands. (A) 1E9 and its TSA (gray) superimposed with
1E9dm binding progesterone (cyan). This view is rotated around
the z axis ≈90° compared with B–D to demonstrate the
movement of the Trp^H50 side chain in 1E9dm caused by the
Leu^H47Trp mutation. (B) The TSA bound by 1E9 (gray) and
5β-androstane-3,17-dione bound by 1E9dm (orange). (C)
Progesterone bound by 1E9dm in the inverse head-to-tail binding
mode with a buried A ring (cyan). The same steroid bound by DB3
(gray). (D) 5β-androstane-3,17-dione bound by 1E9dm (orange)
and DB3 (gray).
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