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Figure 4.
Par-3 enriches PTEN at the junctional membranes via its PDZ3
domain. A, overexpressed Par-3 was specifically enriched at the
junctional membrane in polarized epithelial cells, and PTEN was
partially colocalized with Par-3 (white arrows). In this
experiment, MDCK II cells were transiently co-transfected with
GFP-Par-3 and Myc-PTEN. B, C-terminal tail deletion mutant of
PTEN failed to colocalize with Par-3 at the junctional membrane
regions (arrowhead). C, the DED3A mutant of PTEN also failed to
colocalize with Par-3 at the membrane junctions (arrowhead).
Instead, the PTEN mutant is diffused in the cytoplasm. D, PDZ2
lipid binding-deficient mutant of Par-3 (K3E) is diffused in
cytoplasm (25). Co-expressed PTEN is also absent at the
junctional membranes. E, the oligomerization-deficient mutant of
Par-3 (ΔNTD) (21) is diffused in MDCK cells. Co-expressed PTEN
is also missing from the junctional membranes. Note that the
Par-3 mutants in D and E retain the PTEN binding capacities.
Scale bar, 10 μm. WT, wild type.
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