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Figure 4.
Structures of the Ternary Complex, and H3K4me-Binding
Cavities (A) Molecular surface representation of hPHD-HD1
binding to H3K4me2 (in yellow cylinder style), with W366 and
other critical residues labeled. (B and C) Cylinder
representations of (B) semiaromatic K4me2 cavity and (C) A1
cavity, with critical H bonds indicated as dotted lines and
hydrophobic contacts as double brackets. (D) Molecular surface
representation of PHD, revealing solvent exposure of R2
(regardless of its methylation status). H3K4me, yellow; PHD
cavity residues, green. Note that Tern2 has essentially the same
structure as Tern1 (shown here; see text). (E) Binding constants
of various hPHD point mutants for H3K4me3 15-mer (K[d] values in
[mu]M; see also Figure 3 Figure 3- ).
Mol Cell. 2008 May 23; 30(4-2): 507–518. doi:
10.1016/j.molcel.2008.03.011. Copyright [copyright] 2008 ELL &
Excerpta Medica
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