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Figure 4.
Fig. 4. Low-affinity binding of CD1d-PBS-74 to recombinant
TCR Vα14/2Cβ. (a) Successive dilutions of empty CD1d or
CD1d-PBS-74 were injected over immobilized TCR. Subtraction
(CD1-d-PBS-74 sensorgrams minus empty CD1 sensorgrams) and a 1:1
Langmuir fit of CD1d-PBS-74 binding are presented. (b) Magnified
view of the dissociation phase comparing empty CD1d and
CD1d-PBS-74. (c) Association constants of CD1d-PBS-25 and
CD1d-PBS-74 for Vα14/2Cβ TCR. Measurements were reproduced in
two separate experiments.
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