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Figure 4.
FIGURE 4. The Otu1-ubiquitin interface. A, three regions of
interaction between Otu1 and ubiquitin. The figure is rendered
in a surface representation and shown in "open book" format
highlighting contact regions 1 (blue), 2 (green), and 3 (red).
B, close-up of Otu1-ubiquitin interactions in region 1. Side
chains that mediate hydrogen bonds (dotted line) and van der
Waals interactions are shown. C, close-up of Otu1-ubiquitin
interactions in region 2. Water molecules are indicated as white
spheres. D, close-up of Otu1-ubiquitin interactions in region 3.
E, superimposed active sites of deubiquitinating enzymes. The
catalytic triad cysteine, histidine, and aspartate as well as
ubiquitin (magenta) are shown as a stick models. Otu1 is shown
as cyan, UCH-L3 (Protein Data Bank code 1xd3) is shown in
violet, and HAUSP/USP7 (Protein Data Bank code 1nbf) is shown in
green.
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