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Figure 4.
Fig. 4. Interactions between AtCBL2 and the regulatory domain
of AtCIPK14. (a) The N-lobe, C-lobe, and C-terminal region
interacting with the NAF/FISL motif are in yellow, green, and
blue, respectively. The NAF/FISL motif is represented as a red
ribbon. The NAF/FISL motif binds along the crevice of AtCBL2.
Dashed lines correspond to hydrogen bonds. (b) Close-up view of
the interaction between the CBL–NAF/FISL motif and the
regulatory domain. Hydrogen bonds are shown with dashed lines
(stereo). (c) Pull-down assay of His-tagged AtCBL2 with the
regulatory domain of AtCIPK14 of wild type and mutants.
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