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Figure 4.
Fig. 4. Siglec-5 in complex with (a) α(2,3)-sialyllactose
and (b) α(2,6)-sialyllactose. Sections of the unbiased (before
the inclusion of the ligand) |F[o]| − |F[c]| electron density
maps contoured at 2 σ are shown in the left panels. Siglec-5 is
shown in a ribbon diagram (green), with the Arg124 depicted in
the balls and sticks. The carbohydrate moieties are in yellow.
Interactions between the respective receptor and carbohydrate at
the binding site are shown in the right panels. Direct
protein–ligand interactions with the Sia and the galactose are
shown in red and blue, respectively. Water-mediated interactions
are in green. (c) Superposition of the ligand binding sites of
the sialoadhesin (gray) and Siglec-5 (green) in their complex
structures with α(2,3)-sialyllactose. Residues corresponding to
sialoadhesin are given in parentheses.
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