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Figure 4.
Figure 4. Amino Acid Sequence Conservation at the Surface of
Cia1
(A) Location of the residues exchanged by
site-directed mutagenesis (see Figure 2) from a top view looking
down the central propeller axis and a bottom view obtained by
rotation of 180°.
(B) Representation of the
conservation of amino acid residues in Cia1 from top and bottom
views. The degree of conservation is indicated by the intensity
of the red color. The position of the crucial residue R127 is
highlighted in purple. Amino acid sequences (67 in total) of
Cia1 homologs were collected from various databases by blastp
and tblastn searches. After CLUSTAL W alignment (Thompson et
al., 1994), the degree of conservation was calculated by using
the program ConSurf (Landau et al., 2005). Red, orange, and
light-orange colors correspond to residues with a conservation
score above the 9^th, between the 9^th and 8^th, and between the
8^th and 7^th decile, respectively.
(C) Same as in (B), but
for the side views. The yellow markers in (B) and (C) indicate
the respective sides of the Cia1 structure. The figure was
prepared by using PyMol (http://www.pymol.org) (DeLano, 2002).
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