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Figure 4.
FIGURE 4. Crystal structure of the EDD UBA-ubiquitin
complex. A, overall structure of the complex. Ubiquitin is in
yellow, and the UBA domain is in purple. B, UBA Val^196 binds
the hydrophobic groove formed by Ile^44, His^68, and Val^70 of
ubiquitin. C, UBA Leu^224 binds the groove formed by Ile^44,
Arg^42, Gln^49, and Val^70 of ubiquitin. D, Arg^42 and Gln^49 of
ubiquitin form a network of intermolecular hydrogen bonds with
Asn^221 and Ser^225 of EDD UBA. E, amide of Gly^47 of ubiquitin
forms an intermolecular hydrogen bond with carbonyl of Val^216
of EDD UBA via a bound water molecule. F, bound water molecule
facilitates hydrogen bonding between amide of Leu^71 of
ubiquitin and side chain of Asn^217 of EDD UBA. The 2F[o]-F[c]
electron density (1  contour, omit map) for
bound water molecules is shown in panels E and F.
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