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Figure 4.
Figure 4. Structure of the Calponin Homology Domains of EB1
and Bim1p
Ribbon diagram of the N-terminal CH domain from
EB1 (A) and Bim1p (B) centered on the highly conserved α6
helix. (C) Least-squares fit of EB1 (color) and Bim1p (gray) CH
domains with cylindrical helix representation showing the
overall structural conservation between these two members. (D)
2F[o] − F[c] electron density map at 1.25 Å resolution
of the EB1 CH domain structure contoured at 1.0 σ showing the
highly conserved aromatic core. (E) Ninety degrees rotation of
the EB1 CH domain about the y axis shown above in ribbon format
for orientation and below in CPK representation for conservation
mapping and to summarize mutagenesis results. Center: CH domain
residues with 80% identity across species are represented in
green, 80% conservation in yellow (D and E) (see Figure S3).
Bottom row: results of CH domain mutagenesis on the ability of
EB1[1–187]-LZ to plus end track are mapped. Ablation of
microtubule association, brick red (three cluster mutants
indicated: [S16A, R17E, H18E, D19R], [K59E, K60E], and [K66E,
L67D]). No effect on microtubule plus end tracking, slate.
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