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Figure 4.
Figure 4: Rad4 undergoes conformational changes on DNA-binding.
a, The Rad4–Rad23–DNA complex (red) is superimposed on
the apo-Rad4–Rad23 complex (green) by aligning the TGDs. Black
arrows indicate the movement of BHD1, BHD2 and BHD3 towards the
DNA in the DNA-bound structure relative to the apo-Rad4–Rad23
structure. The BHD3 tip in the apo-Rad4–Rad23 structure is
disordered and is not shown. The Rad23 R4BD is omitted for
clarity. b, Model of undamaged B-type dsDNA bound to Rad4
showing that the apo-Rad4 but not the DNA-bound Rad4
conformation would allow binding to undamaged dsDNA. The model
was prepared by superimposing a B-type dsDNA on the undamaged
dsDNA portion of the Rad4–Rad23–DNA structure.
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