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Figure 4.
(a–e) COS-7 cells were transfected with constructs
expressing YFP or the indicated fluorescent CLIP170 fusions and
stained for endogenous p150^glued. Schematic representations of
CLIP170 fusions are shown at left; CAP-Gly domains (CG1, CG2),
zinc knuckles (Zn1, Zn2), DDETF motif and coiled-coil region are
indicated. Right images show overlays of YFP fluorescence
(green) and staining of endogenous p150^glued (magenta). Inset
in the middle panel in a shows an enlargement of the boxed area
to illustrate localization of p150^glued to microtubule tips in
control cells. Bar, 10  m.
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