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Figure 4.
Figure 4. Superposition of the KUNV (red), DENV (blue) and
YFV (yellow) helicase structures. (a) The Walker A motif
(P-loop, in domain I), and motif V in domain II flank the ATP
binding pocket in the flaviviral helicases (stereo view). In
particular, the Walker A motif in KUNV NS3:186–619 adopts a
conformation that partially closes the ATP binding cavity,
filling the space occupied by ADP α and β-phosphate groups in
the structure of ADP-bound YFV helicase. (b) Details of the
separation between the α2 helix, in domain II, and the α9
helix, in domain III, displayed for the three flaviviral
helicase structures (color-coded as in (a)). The access site for
ssRNA in the protein central cleft is proposed to be located
between these two α-helices.
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