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Figure 5.
Figure 5. Dimer structure. (a) Structure of the TthDEAD
dimer found in the asymmetric unit of crystal form 2. The
monomers are related by an approximate 2-fold (176.5°)
rotation. Helices and β-strands are colored orange and blue,
respectively. Much of the interface is created by an
intermolecular 14-stranded β-sheet, which is an antiparallel
arrangement of the parallel sheets in each monomer. (b) Rotation
of the view in (a) by 90° around the horizontal axis to
highlight additional interactions between the N termini, parts
of the Q-motifs, and the α2 helices of both monomers. (c)
Magnification of the TthDEAD dimer interface in the same
orientation as in (a). The five hydrogen bonds between the β7
strands are shown as broken blue lines. Residues involved in van
der Waals contacts are colored green (molecule A) and cyan
(molecule B). (d) Superposition of the TthDEAD (colored) and
MjaDEAD (grey) dimers. The first molecules of each dimer were
aligned to highlight differences in the other monomers. The mode
of dimerization is similar in both cases via β-strand 7 but the
dimer interface is more extensive in TthDEAD. Note the
difference in tilt between the monomers of each complex (arrow).
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