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Figure 4.
Figure 4. Binding of the WRPW and eh1 Peptide Motifs to the
TLE-WD Domain
(A) Backbone of the SMWRPW peptide (purple)
from human Hes1 on TLE-WD (ribbons) showing that binding occurs
across the pore.
(B) Close-up view of the WPRW core
revealing its compact orientation.
(C) Detailed
intermolecular interactions between the WRPW core and adjacent
amino acids in the WD domain. L743, corresponding to the
aminoacid mutated in MB31, is underlined.
(D) The eh1
peptide backbone (purple) from human Gsc on TLE-WD (ribbons)
indicating the site of peptide binding and its α-helical
structure.
(E) Close-up view of the eh1 core heptapeptide
showing its elongated structure.
(F) Detailed
intermolecular interactions between the core peptide and
adjacent aminoacids in the WD domain showing that contacts are
made to the similar amino acids as for WRPW (C).
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