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Figure 4.
Figure 4 C-domain architecture and HVR. (A) The C-domain
architecture in stereo. The C[w]- and C[h]-domains are in gray
and light green, respectively. The disulfide bridges and the
residues forming the hydrophobic ridges are indicated. The HVR
and its NCS counterpart are shown in red and blue, respectively.
The variable loop (residues 539–549), flanked by two adjacent
cysteine residues, is in green. (B) Crystal packing in the
orthorhombic crystal. The crystallographically equivalent
molecules (HVRs) are in cyan (blue) and pink (red),
respectively. The arrows indicate the directions of the HVR
chains. Zinc and calcium ions are represented as red and black
spheres, respectively. (C) Interactions between the HVRs (cyan
and pink) in stereo. The molecular surface of the cyan molecule
is shown with the electrochemical surface potential (red to
blue). The residues constituting the hydrophobic ridges are in
yellow. The residues are labelled in blue and red for cyan and
pink, respectively. (D) Water-mediated hydrogen-bond network in
the HVR. The HVR residues are in pink and cyan; non-HVR residues
in the pink molecule are in gray.
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