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Figure 4.
(a–e) Electron-density features around the imidazole ring
of His447 at pH 4.5 (a), pH 5.2 (b), pH 5.8 (c), pH 7.3 (d) and
pH 9.0 (e). (f) A view of the interactions between His447,
Asn321 and Asn323 at pH 5.2. The 2F[o] – F[c] density
(magenta) is contoured at 4.0  ,
and the sharpened F[o] – F[c] density (green) is contoured at
2.0 .
The atoms are depicted by a ball-and-stick representation. The
bifurcated hydrogen bonds formed by ND1 of His447 with the amide
groups of Asn321 and Asn323 are drawn as blue dashed lines. The
chemical structure of the histidine side chain with labeled
atoms is included.
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