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Figure 4.
Fig. 4. Assembly mutants prevent the localization of Shank to
the synapse. Visualization of Shank variant distributions in
transfected hippocampal neurons is shown. The wild-type
Shank-416-GFP construct (green) clusters in neuronal dendrites
and colocalizes with Bassoon (red) in transfected hippocampal
neurons (insets: left, GFP; middle, Bassoon; right, merge). In
contrast, mutants (W5E, H22A, M56E, and all three double
mutants) display a diffusely distributed green fluorescence,
indicating no preferential localization to synaptic sites as
indicated by Bassoon staining (insets). F8E shows partial
clustering and partial colocalization with Bassoon (inset).
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