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Figure 4.
Titration of C4BP12 with M4-N. Overlay of five ^15N-^1H HSQC
spectra of 20 μmC4BP12 (20 mm NaOAc, pH 6.0, 37 °C) with
increasing concentrations of M4-N. Colors are as follows: black,
0 μm M4-N; red, 10 μm M4-N; purple, 25 μm M4-N; blue, 50 μm
M4-N; navy, 100 μm M4-N (all concentrations are for the dimer).
Cross-peaks from residues altered in the mutagenesis studies are
labeled.
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