|
Figure 4.
Figure 4. Conservation of the IP20-binding site. Structures
of the wild-type apoenzyme (PDB ID 1J3H [PDB]
; yellow sticks), the E230Q mutant (red sticks), and the ternary
complex of wild-type protein with MgADP-aluminum fluoride and
substrate peptide SP20 (PDB ID 1L3R [PDB]
; dark thin sticks) were superimposed. Some of the negatively
charged residues involved in recognition of the arginines in the
substrate peptide are shown in sticks. SP20 from the ternary
structure (1L3R) is shown in ribbon diagram with P-site Ser,
P-2, P-3, and P-6 Args rendered as ball-and-sticks. Replacement
of Glu230 by Gln did not change its conformation, while its
binding partner Arg133 is disordered.
|
