|
Figure 4.
Figure 4. Structures of TPR1 and TPR2, Domains that Are
Specific to Protein-Primed DNA Polymerases(A) TPR1 forms a
compact domain. This region is an insertion between the palm and
the fingers subdomains. The motif, identified on the basis of
sequence analysis (residues 302–358, gold), can be extended to
include residues 261–301 as well (brown), thereby forming a
subdomain with no homology to the palm subdomains of other B
family polymerases.(B) Structural analogy between TPR2 (cyan)
and the specificity loop (gold) of T7 RNA polymerase. The
fragments of both palms used for superposition are colored in
pink (φ29 DNA polymerase) and gray (T7 RNA polymerase). The
atoms of the residues containing the catalytic carboxylates are
shown as space-filling spheres.
|
