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Figure 4.
Fig. 4. Surface properties of SUMO proteins. The molecular
surface of SUMO-2 (type I crystal) is shown in (A) and (C); that
of the SUMO-1 model is shown in (B) and (D). The charge
potentials in (A) (C) and (D) are calculated using GRASP with a
range of –10 to +10 k[B]T, in which k[B] is Boltzmann constant
and T is Kelvin temperature, and coloured from red to blue.
Neutral areas are shown in white. In (B) the conserved regions
that interact with Ubc9 and Ulp1 are highlighted and coloured in
orange, cyan and magenta, as in Fig. 1. In (E) and (F) the
corresponding amino acids for different surface charges on
SUMO-2 and SUMO-1 are shown. Positively charged, negative
charged and neutral polar residues are coloured blue, red and
magenta, respectively, and nonpolar residues are shown in green.
The views in (C–F) are similar to that of Fig. 3A and those of
(A) and (B) are rotated 180° about the horizontal axis.
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