Figure 4 - full size

Figure 4. Figure 4. Crystal structure of the rat synaptotagmin 4 C[2]B domain. (a) Ribbon diagram of the rat synaptotagmin 4 C[2]B domain. Strands (cyan) are labeled 1 -8 and the single helix (orange) is labeled HA. (b) Electron density map of the Ca^2+-binding site of the rat synaptotagmin 4 C[2]B domain. Contours are drawn at 1.0 the r.m.s. level of the map. Oxygen atoms are red, nitrogen atoms are blue and carbon atoms are yellow. The Ca^2+ ion is labeled Ca, the protein Ca^2+ ligands are labeled D2, D3 and E4, and a coordinating water molecule is labeled W. (c) Backbone superposition of the structures of the rat synaptotagmin 4 C[2]B domain (orange) and the rat synaptotagmin 1 C[2]B domain8 (cyan). (d) Superposition of the Ca^2+-binding loops of the rat synaptotagmin 4 C[2]B domain (orange) and the rat synaptotagmin 1 C[2]B domain (cyan). (e) Superposition of the Ca^2+-binding loops of the rat synaptotagmin 4 C[2]B domain (orange) and the rat synaptotagmin 1 C[2]A domain (dark blue). In d and e, the Ca^2+ ions are in the same color as the protein. The figure was generated with InsightII (MSI) and MolScript35.

The above figure is reprinted by permission from Macmillan Publishers Ltd: Nat Struct Mol Biol (2004, 11, 844-849) copyright 2004.