|
Figure 4.
Figure 4 The PYM -Mago -Y14 complex is conserved across species.
(A) The interaction surfaces of D. melanogaster PYM and the Mago
-Y14 heterodimer have been opened up relative to the view in Fig
2A. The two surfaces are coloured according to sequence
conservation, ranging from orange for conserved residues to
white for variable residues. On the right, the atomic model of
PYM is shown bound to the surface of Mago -Y14. (B) Lysates
prepared from E. coli expressing untagged H. sapiens (Hs) PYM
were incubated with glutathione agarose beads coated with GST,
GST -Hs Y14, GST -Hs Mago or GST -Hs Y14 -Mago dimers.
|
