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Figure 4.
Figure 4. Time-course for glutamylation of E. coli
tRNA[2]^Gln by GlnRS. The inset shows a thin-layer
chromatography plate in which misacylated Glu-AMP is formed to
approximately 50% aminoacylation levels (see Materials and
Methods for details). The % aminoacylation on the ordinate is
derived from the ratio of intensities of the spots corresponding
to Glu-AMP and AMP (right).
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