|
Figure 4.
Fig. 4. Interaction of  -catenin (
-cat) with
F-actin. A, schematic representation of -catenin
and the constructs used in the binding assay. Vinculin homology
regions are shown in dark gray. B, binding to F-actin was
examined by cosedimentation. Assuming a binding ratio of
protein:monomeric actin of 1:7, all -catenin
constructs were added in excess. The supernatant (S) containing
the unbound protein and the pellet (P) containing F-actin and
bound protein are shown for each sample. Molecular mass markers
are indicated on the left.
|
